Effects of inhibitors of signal transduction pathways on transforming growth factor Β1 and osteogenic protein-1-induced insulinlike growth factor binding protein-3 expression in human bone cells

1997 ◽  
Vol 173 (1) ◽  
pp. 28-35 ◽  
Author(s):  
Narasimhan Srinivasan ◽  
David J. Baylink ◽  
Kuber Sampath ◽  
Subburaman Mohan
Keyword(s):  
Signal Transduction ◽  
Growth Factor ◽  
Transforming Growth Factor ◽  
Bone Cells ◽  
Binding Protein ◽  
Transforming Growth Factor Β1 ◽  
Human Bone ◽  
Signal Transduction Pathways ◽  
Factor Binding ◽  
Osteogenic Protein

scholarly journals Transforming growth factor-β1 modulates insulin-like growth factor binding protein-4 expression and proteolysis in cultured periosteal explants

2010 ◽  
Vol 20 (2) ◽  
pp. 81-86 ◽  
Author(s):  
Carlos Gonzalez ◽  
Kiem G. Auw Yang ◽  
Joseph H. Schwab ◽  
James S. Fitzsimmons ◽  
Monica M. Reinholz ◽  
...  
Keyword(s):  
Growth Factor ◽  
Transforming Growth Factor ◽  
Binding Protein ◽  
Transforming Growth Factor Β1 ◽  
Insulin Like Growth Factor ◽  
Factor Binding

Fibrillin-containing microfibrils of normal and regenerating skin contain latent transforming growth factor- binding protein-1 (LTBP-1) and are a repository for latent TGF-β1

1998 ◽  
Vol 16 ◽  
pp. S155
Author(s):  
Michael Raghunath ◽  
Mathias Tschödrich-Rotter ◽  
Christine Unsöld ◽  
Reiner Peters ◽  
Leena Bruckner-Tuderman ◽  
...  
Keyword(s):  
Growth Factor ◽  
Transforming Growth Factor ◽  
Binding Protein ◽  
Factor Binding ◽  
Tgf Β1

scholarly journals Transforming growth factor-β1 up-regulates connexin43 expression in osteocytes via canonical Smad-dependent signaling pathway

2018 ◽  
Vol 38 (6) ◽  
Author(s):  
Wenjing Liu ◽  
Yujia Cui ◽  
Jianxun Sun ◽  
Linyi Cai ◽  
Jing Xie ◽  
...  
Keyword(s):  
Growth Factor ◽  
Connexin 43 ◽  
Transforming Growth Factor ◽  
Bone Cells ◽  
Underlying Mechanism ◽  
Transforming Growth Factor Β1 ◽  
Type I ◽  
Gap Junctional Intercellular Communication ◽  
Cx43 Expression ◽  
Tgf Β1

Connexin 43 (Cx43)-mediated gap junctional intercellular communication (GJIC) has been shown to be important in regulating multiple functions of bone cells. Transforming growth factor-β1 (TGF-β1) exhibited controversial effects on the expression of Cx43 in different cell types. To date, the effect of TGF-β1 on the Cx43 expression of osteocytes is still unknown. In the present study, we detected the expression of TGF-β1 in osteocytes and bone tissue, and then used recombinant mouse TGF-β1 to elucidate its effect on gap junctions (GJs) of osteocytes. Our data indicated that TGF-β1 up-regulated both mRNA and protein expression of Cx43 in osteocytes. Together with down-regulation of Cx43 expression after being treated with TGF-β type I receptor inhibitor Repsox, we deduced that TGF-β1 can positively regulate Cx43 expression in osteocytes. Thus we next focussed on the downstream signals of TGF-β and found that TGF-β1-mediated smads, Smad3 and Smad4, to translocate into nucleus. These translocated signal proteins bind to the promoter of Gja1 which was responsible for the changed expression of Cx43. The present study provides evidence that TGF-β1 can enhance GJIC between osteocytes through up-regulating Cx43 expression and the underlying mechanism involved in the activation of Smad-dependent pathway.

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